GoBio™ Mini A pre-packed, ready to use columns available in 1 ml and 5 ml. Products allow quick, easy and convenient purification of monoclonal and polyclonal antibodies.
GoBio Mini A columns are packed with WorkBeadsTM Protein A resin. The resin is based on agarose from a cross-linking method that results in a highly porous and physically stable agarose matrix. Agarose matrices have successfully been used for many years in biotechnology research and in the industrial purification of proteins. Agarose is proven to be exceptionally compatible with natural biomolecules like proteins, DNA, carbohydrates etc. The material shows minimal non-specific interaction due to the hydrophilic nature of agarose.
Recombinant Protein A is coupled to the matrix with a coupling method giving high chemical stability and low ligand leakage. The recombinant protein A is produced in E. coli and is engineered to facilitate an oriented coupling giving a higher binding capacity. The specificity of the recombinant protein A for the Fc region of IgG provides excellent purification. No animal derived ingredients are used in the fermentation process or the purification of the protein. The high capacity, chemical stability and an established agarose base matrix makes GoBio Mini A ideal for purification of monoclonal antibodies.