GoBio Screen

GoBio Screen

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GoBio™ Screen 7x100 prepacked columns are convenient for fast and easy optimization of methods and parameters, such as selectivity, binding and elution conditions, as well as for small-scale purifications. These columns are prepacked with WorkBeads™ resins which allows quick and easy purification.

Table 1. GoBio Screen 7x100 column characteristics

Column characteristics


Column hardware

Acrylic

Top and bottom plugs

Polypropylene

Top and bottom filters

Polyamide

Connections

1/16" female thread in both ends

Column volume

3.8 mL

Column dimension

7 x 100 mm
Maximal column hardware pressure1

5 bar, 0.5 MPa, 70 psi

¹ The maximum pressure the packed bed can withstand depends on the sample/liquid viscosity and chromatography resin characteristics. The pressure also depends on the tubing used to connect the column and the system restrictions after the column outlet.

Scale-up 
After developing a chromatographic procedure using GoBio Prep 16x100 or GoBio Prep 26x100 column, the column volume can easily be scaled-up by using larger prepacked columns such as, GoBio Prep 16x100 (20 mL), GoBio Prep 26x100 (53 mL) and GoBio Prod 80x200 (1 L). The GoBio Prod prepacked column family is available in column sizes up to 21.4 L.

Table 2. Example of scale-up parameters

Column dimension (mm)

Residence time (minutes)

Linear flow rate (cm/h)

Volumetric flow rate (mL/min)

16×100 4 150 5.0
26×100 4 150 13.3
80×200 8 150 126
130×200 8 150 332
200×200 8 150 785
240×200 8 150 1131
330×250 10 150 2138

 

 

 

 

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