WorkBeads 100S

WorkBeads 100S

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WorkBeads™ 100S resin for ion exchange chromatography is designed for industrial purification applications, which have high flow rate and low back pressure requirements. The product is intended for the purification of proteins and peptides by utilizing the difference in surface charge. WorkBeads 100S is a strong cation exchanger.

  • High throughput and scalability.
  • Reliable and reproducible results.
  • High chemical stability for easy cleaning-in-place.

 

Product

WorkBeads 100S is an agarose-based chromatographic resin manufactured using a proprietary method that results in porous beads with a tight size distribution and exceptional mechanical stability. Agarose based matrices have been successfully used for decades in biotechnology purification, from research to production scale, due to their exceptional compatibility with biomolecules including proteins, peptides, nucleic acids and carbohydrates. WorkBeads resins are designed for separations requiring optimal capacity and purity. WorkBeads 100S is a strong cation exchanger derivatized with sulfonate as functional groups.

 

Table 1. Main characteristics of WorkBeads 100S

Target substances Protein, peptides, 
Matrix Rigid, highly cross-linked agarose
Average particle size¹ (Dv50) 90 - 110 µm
Ionic group (ligand) Sulfonate (-SO₃⁻)
Ionic capacity  180 – 250 µmol H/ml resin
Dynamic binding capacity (DBC)

>100 mg BSA/ml resin²

Pressure flow characteristic

2 bat at 900 cm/h, 25 mm diameter column, 20cm bed height 
Chemical stability Compatible with all standard aqueous buffers used for protein purification, 1 M NaOH, 30% isopropanol and 70% ethanol. Should not be stored at < pH 3 for prolonged time
pH stability 2 – 13
Storage 2 to 25 °C in 20% ethanol with 0.2 M sodium acetate

¹. The median particle size of the cumulative volume distribution.
². Dynamic binding capacity determined at 4-minutes residence time in the presence of 20 mM Na-citrate, pH 4.0.
³. Dynamic binding capacity determined at 4-minutes residence time in the presence of 50 mM Tris-HCl, 50 mM NaCl, pH 8.0.

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