WorkBeads™ 100S resin for ion exchange chromatography is designed for industrial purification applications, which have high flow rate and low back pressure requirements. The product is intended for the purification of proteins and peptides by utilizing the difference in surface charge. WorkBeads 100S is a strong cation exchanger.
WorkBeads 100S is an agarose-based chromatographic resin manufactured using a proprietary method that results in porous beads with a tight size distribution and exceptional mechanical stability. Agarose based matrices have been successfully used for decades in biotechnology purification, from research to production scale, due to their exceptional compatibility with biomolecules including proteins, peptides, nucleic acids and carbohydrates. WorkBeads resins are designed for separations requiring optimal capacity and purity. WorkBeads 100S is a strong cation exchanger derivatized with sulfonate as functional groups.
Table 1. Main characteristics of WorkBeads 100S
|Target substances||Protein, peptides,|
|Matrix||Rigid, highly cross-linked agarose|
|Average particle size¹(Dv50)||90 - 110 µm|
|Ionic group (ligand)||Sulfonate (-SO₃⁻)
|Ionic capacity||180 – 250 µmol H⁺/ml resin|
|Dynamic binding capacity (DBC)||
>100 mg BSA/ml resin²
Pressure flow characteristic
|2 bat at 900 cm/h, 25 mm diameter column, 20cm bed height|
|Chemical stability||Compatible with all standard aqueous buffers used for protein purification, 1 M NaOH, 30% isopropanol and 70% ethanol. Should not be stored at < pH 3 for prolonged time|
|pH stability||2 – 13|
|Storage||2 to 25 °C in 20% ethanol with 0.2 M sodium acetate|
¹. The median particle size of the cumulative volume distribution.
². Dynamic binding capacity determined at 4-minutes residence time in the presence of 20 mM Na-citrate, pH 4.0.
³. Dynamic binding capacity determined at 4-minutes residence time in the presence of 50 mM Tris-HCl, 50 mM NaCl, pH 8.0.