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WorkBeads™ affimAb is an optimized alkali-stable Protein A resin designed for the purification of monoclonal- and polyclonal antibodies in laboratory to process scale development. This resin has a superior base matrix in combination with an optimized alkali-stable Protein A ligand. This results in high dynamic binding capacity also at short residence times, and stabile capacity over multiple purification cycles with cleaning-in-place using 0.5 M NaOH.
- Top performance dynamic binding capacity also at short residence times
- Outstanding alkali stability with 0.5 M NaOH, extends the numbers of purification cycles
- Excellent purity, recovery and reproducibility
- Negligible Protein A leakage
A resin for affinity chromatography
An expanding mAb market has driven the need for highly efficient Protein A resins. Compared to the market-leading resin, WorkBeads affimAb resin allows for higher purity of eluted mAbs from cell supernatants. The affinity chromatography resin allows mAb purification that gives exceptionally low host cell protein (HCP) and DNA (HCD) levels. Prepacked GoBio™ affimAb columns are available from 1 mL to 21.4 L columns for small scale purifications to production scale. WorkBeads affimAb resin can also be used for purifications in other formats, such as batch and centrifugation purifications.
WorkBeads are agarose-based chromatographic resins manufactured using a proprietary method that results in porous beads with tight size distribution and exceptional mechanical stability. WorkBeads resins are designed for separations requiring optimal capacity and purity.
Table 1. Main characteristics of WorkBeads affimAb resin
| WorkBeads affimAb | |
| Target substances | Antibodies (IgG), bound via the Fc-region |
| Matrix | Rigid, highly cross-linked agarose |
| Average particle size (Dv50)¹ | 50 µm |
| Ligand | Recombinant Protein A expressed in E. coli using animal-free medium |
| Dynamic binding capacity (DBC)² | > 40 mg human IgG/mL resin |
| Max flow rate³ (20 cm bed height, 5 bar) |
300 cm/h |
| Chemical stability | Compatible with all standard aqueous buffers used for protein purification, 10 mM HCl (pH 2), 0.5 M NaOH (pH 12), 0.1 M sodium citrate buffer (pH 3), 6 M guanidine-HCl, 20% ethanol. Note: Should not be stored at low pH for prolonged time. |
| pH stability | 3 – 12 |
| Cleaning-in-place stability | Up to 0.5 M NaOH |
| Storage | 2 to 8 °C in 20% ethanol |
¹ The median particle size of the cumulative volume distribution.
² Dynamic binding capacity was determined at 10% breakthrough (QB10%) by frontal analysis with 1 mg/mL human polyclonal lgG in PBS, pH 7.4 at 1.4 mL/min (240 cm/h, 2.5 minutes residence time) in a 6.6 × 100 mm column.
³ Max recommended flow rate at 20 °C using aqueous buffers. Decrease the max flow rate if the liquid has a higher viscosity. Higher viscosities can be caused by low temperature (use half of the max flow rate when operating at 4 °C), or by additives (e.g., use half of the max flow rate for 20% ethanol).