WorkBeads™ 40Q resin for ion exchange chromatography is designed for research and industrial scale purification of proteins, peptides and oligonucleotides by utilizing the difference in their surface charge. WorkBeads 40Q resin is a strong anion exchanger derivatized with quaternary amine ligands while its contrary WorkBeads 40S is a strong cation exchanger derivatized with sulfonate ligands. These resins demonstrate the property of high-resolution separation while giving low backpressure to facilitate both capture and polishing purification applications in standard bioprocess columns.
WorkBeads 40Q is an agarose-based chromatographic resin manufactured using a proprietary method that results in porous beads with a tight size distribution and exceptional mechanical stability. Agarose-based matrices have been successfully used for decades in biotechnology purification, from research to production scale, due to their exceptional compatibility with biomolecules including proteins, peptides, nucleic acids and carbohydrates. WorkBeads resins are designed for separations requiring optimal capacity and purity. WorkBeads 40Q is a strong anion exchanger derivatized with quarternary amines as functional groups.
Main characteristics of WorkBeads 40Q
|Target substances||Protein, peptides, viruses, oligonucleotides|
|Matrix||Rigid, highly cross-linked agarose|
|Average particle size1 (Dv50)||45 µm|
|Ionic group (ligand)||Quaternary amine (-N+(CH3)3)|
|Ionic capacity||180 – 250 µmol Cl- /mL resin|
|Dynamic binding capacity (DBC)||50 mg BSA/mL resin2|
|Maximum flow rate3
(20 cm bed height and 5 bar)
|Chemical stability||Compatible with all standard aqueous buffers used for protein purification, 1 M NaOH, 30% isopropanol and 70% ethanol. Should not be stored at low pH for prolonged time.|
|Operational pH4||2 – 13|
|CIP and screening pH5||2 – 14|
|Storage||2 to 25 °C in 20% ethanol|
1 The median particle size of the cumulative volume distribution.
2 Dynamic binding capacity determined at 2.5-minutes residence time in 50 mM Tris-HCl, 50 mM NaCl, pH 8.0.
3 Optimal flow rate during binding is depending on the sample.
4 Within the operational pH range, the resin can be operated without significant change in function. Within the CIP (Cleaning-in-place) and screening pH range the resin can be subjected to the denoted pH range without significant change in function.