WorkBeads 40 TREN

WorkBeads™ 40 TREN resin for multimodal Ion Exchange Chromatography (IEX) features a ligand that is positively charged below approximately pH 9. This resin is versatile and can be utilized for various applications, particularly due to its higher salt tolerance. It is especially effective in multimodal IEX, for sample clean-up in monoclonal antibody (mAb) purification processes to protect the Protein A column from chromatins and other host cell impurities, or as a polishing step in the mAb purification process.

• Differential selectivity due to higher salt tolerance and multimodal properties
• Reduced fouling of Protein A resins by removing viruses and host cell impurities
• High binding capacity and enhanced purity

Product
WorkBeads 40 TREN resin contains ligands based on Tris(2-aminoethyl)amine (TAEA). The structure of the ligand used in WorkBeads 40 TREN is shown in Figure 1, see below. WorkBeads 40 TREN resin can be used for the separation of biomolecules exploiting surface charge to purify proteins, peptides and oligonucleotides. It can also be used in flow through mode to adsorb impurities while letting the target pass through the column (negative chromatography mode).

Purification of antibodies from mammalian host cells, such as Chinese Hamster Ovary (CHO), results in extensive bioburden on the Protein A column. Regular cleaning-in-place (CIP) is mandatory in the purification process, but accumulative fouling of the column will still occur.  It would be desirable to add a purification step before the protein A purification step to reduce the components that constitute the worst threat, thereby maximizing the lifetime of the Protein A column and in the process improve the final purity of the target antibody.

WorkBeads 40 TREN has proven effective in removing impurities such as chromatin complexes (nucleic acid-protein complexes), endotoxins, and host cell proteins. The white paper titled Improving Your mAb Purification (click the link on this page to access the document) provides a detailed explanation of how WorkBeads 40 TREN can be integrated upstream of WorkBeads affimAb and utilized in flow-through mode to adsorb unwanted impurities, allowing monoclonal antibodies (mAbs) to pass through the column without binding.

See also blog articles: https://blog.bio-works.com/reversing-bioprocess-steps-ecp and https://blog.bio-works.com/enhanced-capture-process-a-presentation