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The ready-to-use GoBio™ Mini Butyl SH columns are prepacked with WorkBeads™ 40 Butyl SH resin and are available in two column sizes, 1 mL and 5 mL. This resin can be used for several different applications, such as the purification of proteins, peptides, plasmids, and oligonucleotides.
- Prepacked and ready-to-use columns for fast and reliable results
- High throughput, binding capacity, and purity
- Reliable and reproducible results
- High chemical stability for easy cleaning-in-place
A resin for hydrophobic interaction chromatography (HIC)
The functional ligand of WorkBeads 40 Butyl SH is n-butyl thioether. Since butyl is a very hydrophobic linear chain, minimal mixed-mode interactions are expected. The resin is optimized to offer reliable binding performance. The hydrophobic interaction chromatography (HIC) resin is also available in several different prepacked column sizes, such as GoBio™ Mini 1 mL and 5 mL, GoBio Screen 7 x 100 (3.8 mL), GoBio Prep 16 x 100 (20 mL) and 26 x 100 (53 mL) as well as GoBio Prod columns starting at 1 L.
WorkBeads are agarose-based chromatographic resins manufactured using a proprietary method that results in porous beads with tight size distribution and exceptional mechanical stability. WorkBeads resins are designed for separations requiring optimal capacity and purity.
Table 1. Main characteristics of GoBio Mini Butyl SH columns
| GoBio Mini Butyl SH | |
| Target substances | Proteins, peptides, plasmids, oligonucleotides |
| Resin | WorkBeads 40 Butyl SH |
| Matrix | Rigid, highly cross-linked agarose |
| Average particle size (Dv50)¹ | 45 µm |
| Ligand |
n-butyl thioether (CH₃ - CH₂ - CH₂ - CH₂ - S -) |
| Ligand density | 46 – 62 µmol/mL resin |
| Dynamic binding capacity (DBC)² | 43 mg β-lactoglobulin/mL resin |
| Column volume | 1 mL 5 mL |
| Column dimension | 7 × 28 mm (1 mL) 13 × 38 mm (5 mL) |
| Recommended flow rate³ GoBio Mini 1 mL GoBio Mini 5 mL |
0.25 – 1 mL/min (37 – 150 cm/h) 1.25 – 5 mL/min (56 – 225 cm/h) |
| Maximum flow rate⁴ GoBio Mini 1 mL GoBio Mini 5 mL |
5 mL/min (780 cm/h) 20 mL/min (900 cm/h) |
| Maximum back pressure | 0.3 MPa, 3 bar, 43 psi |
| Chemical stability | Compatible with all standard aqueous buffers exhibiting some conductivity, 1 M NaOH, 30% isopropanol, 30% ethanol. Note: Sensitive to oxidants, e.g., H₂O₂. |
| pH stability | 2 – 13 |
| Storage | 2 to 25°C in 20% ethanol |
¹ The median particle size of the cumulative volume distribution.
² Dynamic binding capacity at 10% breakthrough determined at a residence time of 4 min (150 cm/h) in a 6.6x100 mm column. Buffer conditions: 0.1 M sodium phosphate, 2 M ammonium sulfate, pH 7.
³ The optimal flow rate during binding is dependent on the sample. During column wash and elution, a flow rate of 1 mL/min and 5 mL/min can be used for 1 mL and 5 mL columns, respectively. Note: The maximum pressure the packed bed can withstand depends on the sample/liquid viscosity and chromatography resin characteristics. The pressure also depends on the tubing used to connect the column and the system restrictions after the column outlet.
⁴ Aqueous buffers at 20°C. Decrease the maximum flow rate if the liquid has a higher viscosity. Higher viscosities can be caused by low temperature (use half of the maximum flow rate when operating at +4°C) or by additives (e.g., use half of the maximum flow rate for 20% ethanol).