GoBio™ Mini Dsalt pre-packed, ready to use column available in 1 mL and 5 mL which allows quick, easy and convenient group separation of high and low molecular weight substances.
Proteins and other biomolecules differ greatly in size from salts and other small molecules. Size exclusion is an efficient technique to separates the components in high molecular weight substances and low molecular weight substances. The exclusion limit is approximately Mr 5000. Buffer exchange and desalting are common uses in laboratories working with purification and analysis, GoBio Mini Dsalt is excellent for this.
Buffer exchange or desalting of a sample can be used to prepare for mass spectroscopy analysis, lyophilization and after certain procedures such as ion exchange chromatography. GoBio Mini Dsalt is a useful alternative to dialysis when larger sample volumes are used or when samples need to be processed rapidly to avoid degradation.