Pre-packed OptioBio™ 40Q 10x100 glass columns are designed for small-scale purification as well as screening and optimization in bioprocess development and scale-up.
OptioBio 40Q 10x100 column is pre-packed with WorkBeads™ 40Q resin for ion exchange chromatography (IEX). The resin is designed for research and industrial scale purification of proteins, peptides and nucleic acids and utilise the difference in their surface charge. WorkBeads 40Q is a strong anion exchange resin with quaternary amine ligands. WorkBeads are agarose based chromatographic resins manufactured using a proprietary method that results in porous beads with a tight size distribution and high mechanical stability. WorkBeads resins are designed for separations that require optimal capacity and purity.
Table 1. Main characteristics of OptioBio 40S 10x100 columns
|Target substances||Protein, peptides,|
|Matrix||Rigid, highly cross-linked agarose|
|Average particle size1 (Dv50)||45 µm|
|Ionic group (ligand)||Sulfonate (-SO₃⁻)|
|Ionic capacity||180 – 250 µmol H⁺/mL resin|
|Dynamic binding capacity (DBC)||150 mg BSA/mL resin²|
|Column Volume (CV)||7.9 ml|
|Column dimension||10 x 100 mm|
|Recommended flow rate||2 - 4/min (150 - 300/h)|
|Maximum flow rate⁴||6 ml/min (450/h)|
|Column hardware pressure limit||2.1 MPa, 21 bar, 305 psi|
|Chemical stability||Compatible with all standard aqueous buffers used for protein purification, 1 M NaOH, 30% isopropanol and 70% ethanol. Should not be stored at low pH for prolonged time.|
3 - 12 (Working range)
2 - 13 (cleaning)
|Storage||2 to 25 °C in 20% with 0.2 M sodium acetate|
¹. The median particle size of the cumulative volume distribution.
². Dynamic binding capacity determined in 20 mM Na-citrate, pH 4.0, at a flow of 2 ml/min (150 cm/h; 4 minutes residence time).
³. Dynamic binding capacity determined in SO mM Tris-HCI, 50 mM NaCI, pH 8.0, at a flow of 2 ml/min (150 cm/h; 4 minutes residence time).
⁴. Maximum flow rate for aqueous buffers at 20 oc. Decrease the maximum flow rate if the liquid has a higher viscosity. Higher viscosities can be caused by low temperature.
Use half of the maximum flow rate for 20% ethanol