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WorkBeads™ 40 Butyl SH is a resin for hydrophobic interaction chromatography (HIC) designed for research and industrial scale purification of proteins, peptides, plasmids, and oligonucleotides by utilizing the difference in their surface hydrophobicity. It’s often used to complement other techniques that separate according to either charge or size.
- High throughput, binding capacity, and purity
- High chemical stability for easy cleaning-in-place and reproducible results
- Prepacked GoBio columns for convenience and reproducibility
A resin for hydrophobic interaction chromatography (HIC)
The functional ligand of WorkBeads 40 Butyl SH is n-butyl thioether. Since butyl is a very hydrophobic linear chain, minimal mixed-mode interactions are expected. The resin is optimized to offer reliable binding performance. The hydrophobic interaction chromatography (HIC) resin is also available in several different prepacked column sizes, such as GoBio™ Mini 1 mL and 5 mL, GoBio Screen 7 x 100 (3.8 mL), GoBio Prep 16 x 100 (20 mL) and 26 x 100 (53 mL) as well as GoBio Prod columns starting at 1 L.
WorkBeads are agarose-based chromatographic resins manufactured using a proprietary method that results in porous beads with tight size distribution and exceptional mechanical stability. WorkBeads resins are designed for separations requiring optimal capacity and purity.
Table 1. Main characteristics of WorkBeads 40 Butyl SH resin
| WorkBeads 40 Butyl SH | |
| Target substances | Proteins, peptides, plasmids, oligonucleotides |
| Matrix | Rigid, highly cross-linked agarose |
| Average particle size (Dv50)¹ | 45 µm |
| Ligand |
n-butyl thioether (CH₃ - CH₂ - CH₂ - CH₂ - S -) |
| Ligand density | 46 – 62 µmol/mL resin |
| Dynamic binding capacity (DBC)² |
43 mg β-lactoglobulin/mL resin |
| Max flow rate³ (20 cm bed height, 5 bar) |
600 cm/h |
| Chemical stability | Compatible with all standard aqueous buffers exhibiting some conductivity, 1 M NaOH, 30% isopropanol, 30% ethanol. Note: Sensitive to oxidants, e.g., H₂O₂. |
| pH stability | 2 – 13 |
| Storage | 2 to 25°C in 20% ethanol |
¹ The median particle size of the cumulative volume distribution.
² Dynamic binding capacity at 10% breakthrough determined at a residence time of 4 min (150 cm/h) in a 6.6x100 mm column. Buffer conditions: 0.1 M sodium phosphate, 2 M ammonium sulfate, pH 7. The optimal flow rate during binding is dependent on the sample.
³ Decrease the maximum flow rate if the liquid has a higher viscosity. Higher viscosities can be caused by low temperature (use half of the maximum flow rate when operating at +4°C) or by additives (e.g., use half of the maximum flow rate for 20% ethanol).