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WorkBeads™ affimAb resin is an optimized alkali-stable resin designed for the purification of monoclonal antibodies from laboratory to process scale. This resin has a superior base matrix combined with an patented, alkali-stable protein A ligand which results in high dynamic binding capacity at short residence times. The resin is designed to withstand many repeated purification cycles without losing binding capacity even when cleaning with 0.5 M NaOH between each run. For small-scale purifications and initial screening in process development, convenient prepacked GoBio Mini and GoBio Screen columns are available. For larger-scale processes, GoBio Prep and GoBio Prod columns are available in sizes up to 21L.
- Top performance dynamic binding capacity also at short residence times
- Outstanding alkali stability with 0.5 M NaOH, extends the number of purification cycles
- Excellent purity, recovery and reproducibility
- Negligible protein A leakage
- Convenient pre-packed 1 ml and 5 ml BabyBio™ columns
Resin description
WorkBeads are agarose based chromatographic resins manufactured by a proprietary method that results in porous beads with a tight size distribution and very high mechanical stability. Agarose based matrices have been successfully used for decades in biotechnology research from laboratory to production scale, due to their exceptional compatibility with biomolecules including proteins, peptides, nucleic acids and carbohydrates. WorkBeads resins are designed for separations requiring optimal capacity and purity.
The alkali stable recombinant protein A attached to the optimized basematrix is produced in E. coli under conditions free of components of animal origin and purified to high purity before coupling. This combination gives both high dynamic binding capacities for antibodies and the possibility for efficient cleaning-in-place with 0.5 M NaOH.
Characteristics of WorkBeads affimAb
| Target substances |
Antibodies (IgG), bound via the Fc-region |
| Matrix | Rigid, highly cross-linked agarose |
| Average particle size¹ (Dv₅₀) | 50 µm |
| Ligand |
Recombinant protein A expressed in E. coli using animal-free medium |
|
Dynamic binding capacity² (DBC) |
> 40 mg human lgG/ml resin |
|
Max recommended flow rate³ |
300 cm/h |
| Chemical stability |
Compatible with all standard aqueous buffers used for protein purification. Should not be stored at low pH for prolonged time. |
| pH stability | 3 – 10 |
| Cleaning-in-place stability | Up to 0.5 M NaOH |
| Storage | 2 to 8°C in 20 % ethanol |
¹The median particle size of the cumulative volume distribution.
². DBC was determined at 10% breakthrough (QB104) by frontal analysis with 1 mg/ml human polyclonal lgG in PBS, pH 7,4 AT 1,4 ml/min (245 cm/h. 2.5 minutes residence time)
³. Max recommended flow rate at 20°C using acqueous buffers. Decrease the max flow rate if the liquid has a higher viscosity. Higher viscosities can be caused by low temperature (use half of the max flow rate when operating at 4°C), or by additives (e.g., use half of the max flow rate for 20% ethanol)